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Studying Z-DNA and B-to Z-DNA transitions using a cytosine analogue FRET-pair

Blaise Dumat (Institutionen för kemi och kemiteknik) ; Anders Foller Larsen (Institutionen för kemi och kemiteknik) ; Marcus Wilhelmsson (Institutionen för kemi och kemiteknik, Fysikalisk kemi)
Nucleic Acids Research (0305-1048). Vol. 44 (2016), 11,
[Artikel, refereegranskad vetenskaplig]

Herein, we report on the use of a tricyclic cytosine FRET pair, incorporated into DNA with different base pair separations, to study Z-DNA and B-Z DNA junctions. With its position inside the DNA structure, the FRET pair responds to a B- to Z-DNA transition with a distinct change in FRET efficiency for each donor/acceptor configuration allowing reliable structural probing. Moreover, we show how fluorescence spectroscopy and our cytosine analogues can be used to determine rate constants for the B- to Z-DNA transition mechanism. The modified cytosines have little influence on the transition and the FRET pair is thus an easily implemented and virtually non-perturbing fluorescence tool to study Z-DNA. This nucleobase analogue FRET pair represents a valuable addition to the limited number of fluorescence methods available to study Z-DNA and we suggest it will facilitate, for example, deciphering the B- to Z-DNA transition mechanism and investigating the interaction of DNA with Z-DNA binding proteins.



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Denna post skapades 2016-09-09. Senast ändrad 2016-09-09.
CPL Pubid: 241589

 

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