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Methods for the physical characterization and quantification of extracellular vesicles in biological samples

Déborah L. M. Rupert (Institutionen för fysik (Chalmers)) ; Virginia Claudio (Institutionen för fysik, Biologisk fysik (Chalmers) ; Institutionen för neurovetenskap och fysiologi) ; Cecilia Lässer ; Marta Bally (Institutionen för fysik, Biologisk fysik (Chalmers))
Biochimica et Biophysica Acta - General Subjects (0304-4165). Vol. Epub ahead of print (2016),
[Artikel, refereegranskad vetenskaplig]

BACKGROUND: Our body fluids contain a multitude of cell-derived vesicles, secreted by most cell types, commonly referred to as extracellular vesicles. They have attracted considerable attention for their function as intercellular communication vehicles in a broad range of physiological processes and pathological conditions. Extracellular vesicles and especially the smallest type, exosomes, have also generated a lot of excitement in view of their potential as disease biomarkers or as carriers for drug delivery. In this context, state-of-the-art techniques capable of comprehensively characterizing vesicles in biological fluids are urgently needed. SCOPE OF REVIEW: This review presents the arsenal of techniques available for quantification and characterization of physical properties of extracellular vesicles, summarizes their working principles, discusses their advantages and limitations and further illustrates their implementation in vesicle research. MAJOR CONCLUSIONS: The small size and physicochemical heterogeneity of extracellular vesicles make their physical characterization and quantification an extremely challenging task. Currently, structure, size, buoyant density, optical properties and zeta potential have most commonly been studied. The concentration of vesicles in suspension can be expressed in terms of biomolecular or particle content depending on the method at hand. In addition, common quantification methods may either provide a direct quantitative measurement of vesicle concentration or solely allow for relative comparison between samples. GENERAL SIGNIFICANCE: The combination of complementary methods capable of detecting, characterizing and quantifying extracellular vesicles at a single particle level promises to provide new exciting insights into their modes of action and to reveal the existence of vesicle subpopulations fulfilling key biological tasks.

Nyckelord: Exosomes; Extracellular vesicles; Analytical methods; Quantification; Characterization; Physical properties



Denna post skapades 2016-08-17. Senast ändrad 2016-10-04.
CPL Pubid: 240336

 

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Institutioner (Chalmers)

Institutionen för fysik (Chalmers)
Institutionen för fysik, Biologisk fysik (Chalmers)
Institutionen för neurovetenskap och fysiologi (GU)
Krefting Research Centre (GU)

Ämnesområden

Fysik
Cellbiologi
Nanoteknik
Cell- och molekylärbiologi

Chalmers infrastruktur