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Rapid Tracing of Resistance Plasmids in a Nosocomial Outbreak Using Optical DNA Mapping

Vilhelm Müller (Institutionen för biologi och bioteknik, Kemisk biologi) ; Nahid Karami ; Lena Nyberg (Institutionen för biologi och bioteknik, Kemisk biologi) ; C. Pichler ; P. C. T. Pedreschi ; Saair Quaderi (Institutionen för biologi och bioteknik) ; Joachim Fritzsche (Institutionen för fysik, Kemisk fysik (Chalmers)) ; T. Ambjornsson ; Christina Åhrén ; Fredrik Westerlund (Institutionen för biologi och bioteknik, Kemisk biologi)
Acs Infectious Diseases (2373-8227). Vol. 2 (2016), 5, p. 322-328.
[Artikel, refereegranskad vetenskaplig]

Resistance to life-saving antibiotics increases rapidly worldwide, and multiresistant bacteria have become a global threat to human health. Presently, the most serious threat is the increasing spread of Enterobacteriaceae carrying genes coding for extended spectrum beta-lactamases (ESBL) and carbapenemases on highly mobile plasmids. We here demonstrate how optical DNA maps of single plasmids can be used as fingerprints to trace plasmids, for example, during resistance outbreaks. We use the assay to demonstrate a potential transmission route of an ESBL-carrying plasmid between bacterial strains/species and between patients, during a polyclonal outbreak at a neonatal ward at Sahlgrenska University Hospital (Gothenburg, Sweden). Our results demonstrate that optical DNA mapping is an easy and rapid method for detecting the spread of plasmids mediating resistance. With the increasing prevalence of multiresistant bacteria, diagnostic tools that can aid in solving ongoing routes of transmission, in particular in hospital settings, will be of paramount importance.

Nyckelord: optical DNA mapping, plasmids, antibiotic resistance, nosocomial outbreak, field gel-electrophoresis, spectrum beta-lactamases, escherichia-coli, antibiotic-resistance, enterobacteriaceae, molecules, sequence, spread, Pharmacology & Pharmacy, Infectious Diseases

Denna post skapades 2016-08-12. Senast ändrad 2017-06-27.
CPL Pubid: 240187


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Institutioner (Chalmers)

Institutionen för biologi och bioteknik, Kemisk biologi
Institutionen för biomedicin, avdelningen för infektionssjukdomar (GU)
Institutionen för biologi och bioteknik
Institutionen för fysik, Kemisk fysik (Chalmers)


Klinisk medicin

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Optical Mapping of Bacterial Plasmids