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Dynamic and Progressive Control of DNA Origami Conformation by Modulating DNA Helicity with Chemical Adducts

H. R. Chen ; H. Y. Zhang ; J. Pan ; T. G. Cha ; Shiming Li (Institutionen för kemi och kemiteknik, Fysikalisk kemi) ; Joakim Andréasson (Institutionen för kemi och kemiteknik, Fysikalisk kemi) ; J. H. Choi
ACS Nano (1936-0851). Vol. 10 (2016), 5, p. 4989-4996.
[Artikel, refereegranskad vetenskaplig]

DNA origami has received enormous attention for its ability to program complex nanostructures with a few nanometer precision. Dynamic origami structures that change conformation in response to environmental cues or external signals hold great promises in sensing and actuation at the nanoscale. The reconfiguration mechanism of existing dynamic origami structures is mostly limited to single-stranded hinges and relies almost exclusively on DNA hybridization or strand displacement. Here, we show an alternative approach by demonstrating on-demand conformation changes with DNA-binding molecules, which intercalate between base pairs and unwind DNA double helices. The unwinding effect modulates the helicity mismatch in DNA origami, which significantly influences the internal stress and the global conformation of the origami structure. We demonstrate the switching of a polymerized origami nanoribbon between different twisting states and a well-constrained torsional deformation in a monomeric origami shaft. The structural transformation is shown to be reversible, and binding isotherms confirm the reconfiguration mechanism. This approach provides a rapid and reversible means to change DNA origami conformation, which can be used for dynamic and progressive control at the nanoscale.

Nyckelord: DNA origami, DNA binding, conformation change

Denna post skapades 2016-07-04. Senast ändrad 2017-09-14.
CPL Pubid: 238848


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Institutioner (Chalmers)

Institutionen för kemi och kemiteknik, Fysikalisk kemi


Fysikalisk kemi

Chalmers infrastruktur