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Graphene oxide based coatings on nitinol for biomedical implant applications: effectively promote mammalian cell growth but kill bacteria

Changhong Zhao (Institutionen för mikroteknologi och nanovetenskap, Elektronikmaterial och system ) ; Santosh Pandit (Institutionen för biologi och bioteknik, Systembiologi) ; Yifeng Fu (Institutionen för mikroteknologi och nanovetenskap, Elektronikmaterial och system ) ; Ivan Mijakovic (Institutionen för biologi och bioteknik, Systembiologi) ; Aldo Jesorka (Institutionen för kemi och kemiteknik, Fysikalisk kemi) ; Johan Liu (Institutionen för mikroteknologi och nanovetenskap, Elektronikmaterial och system )
RSC Advances (2046-2069). Vol. 6 (2016), 44, p. 38124-38134.
[Artikel, refereegranskad vetenskaplig]

An important clinical challenge is the development of implant surfaces which have good integration with the surrounding tissues and simultaneously inhibit bacterial colonization thus preventing infection. Recently, graphene oxide (GO) a derivative of graphene, has gained considerable attention in the biomedical field owing to its biocompatibility, surface functionalizability and promising antimicrobial activity. In this study gelatin-functionalized graphene oxide (GOGel) was synthesized by a simple one step modification where GO and GOGel were used to develop surface coatings on nitinol substrates. Mouse osteoblastic cell (MC3T3-E1) functions including cell attachment, proliferation and differentiation were investigated on GO-based coatings. The results indicated that MC3T3-E1 cell functions were significantly enhanced on both GO coated nitinol (GO@NiTi) and GOGel coated nitinol (GOGel@NiTi) compared with the control nitinol without coating (NiTi). Especially, the GOGel@NiTi surface exhibited the best performance for cell adhesion, proliferation and differentiation. Additionally the antimicrobial property of GO-based coatings against E. coli was studied with the evaluation of colony forming units (CFU) counting, live/dead fluorescent staining and scanning electron microscope (SEM). We found that the growth of E. coli was inhibited on GOGel@NiTi and particularly on GO@NiTi. SEM images revealed that the cell membrane of bacteria lost their integrity and live/dead fluorescent images confirmed the low live/dead ratio of E. coli after incubation on GOGel@NiTi and GO@NiTi. We conclude that GO-based coatings on NiTi combine the antimicrobial activity and improved biocompatibility and therefore present a remarkable potential in biomedical implant applications.

Nyckelord: protein adsorption, functionalized graphene, surfaces, antibacterial, nanosheets, films, differentiation, cytotoxicity, fibroblasts, behavior

Denna post skapades 2016-06-17. Senast ändrad 2016-07-08.
CPL Pubid: 237886


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