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A multi-purpose microfluidic pipette for single-cell analysis

Alar Ainla (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Erik T. Jansson (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Natalia Stepanyants (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Owe Orwar (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Aldo Jesorka (Institutionen för kemi- och bioteknik, Fysikalisk kemi)
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Groningen; Netherlands; 3 October 2010 through 7 October 2010 Vol. 2 (2010), p. 932-934.
[Konferensbidrag, refereegranskat]

We report a multi-purpose microfluidic pipette, with a recirculating liquid tip. This device, made in poly(dimethylsiloxane), enables contamination-free manipulation and chemical stimulation of selected single cells in cell collectives or tissue slices. The pipette is capable of carrying out a variety of complex fluid processing functionalities, such as mixing, multiplexing, or gradient generation. The concept is flexible and scalable as the geometry and the size of the recirculation zone is defined by pressure, channel number, and geometry. We have applied the pipette in a fluorescence uptake assay, electrophysiology studies and for chemical induction of membrane protrusion from biological cells.

Nyckelord: Microfluidics, Single-cell analysis, Single-cell manipulation, Solution exchange, Superfusion, Chemical induction, Chemical stimulation, Contamination-free, Electrophysiology study, Recirculation zones, Single cells, Singlecell analysis, Electrophysiology, Liquids, Molecular biology



Denna post skapades 2016-05-12.
CPL Pubid: 236456

 

Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)

Ämnesområden

Fysikalisk kemi

Chalmers infrastruktur