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A lipid-based passivation scheme for nanofluidics

Joachim Fritzsche ; F. Persson ; K. U. Mir ; M. Modesti ; Fredrik Westerlund (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Jonas O. Tegenfeldt
16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012; Okinawa; Japan; 28 October 2012 through 1 November 2012 p. 1876-1878. (2012)
[Konferensbidrag, refereegranskat]

Stretching DNA in nanochannels allows for direct, visual studies of genomic DNA at the single molecule level. In order to facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We show long-term passivation of nanochannel surfaces to several relevant reagents and demonstrate that the performance of the lipid bilayer is significantly better compared to standard bovine serum albumin-based passivation. Moreover, we demonstrate how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation.

Nyckelord: Lipid bilayer, Nanofluidics, Passivation, Protein-DNA interactions, Single molecules, DNA, Lipid bilayers, Molecules, Proteins, Bovine serum, DNA cleavage, Enzymatic Degradation, Genomic DNA, Nano channels, Single molecule, Single molecule level



Denna post skapades 2016-05-12.
CPL Pubid: 236451

 

Institutioner (Chalmers)

Institutionen för fysik (GU) (GU)
Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)

Ämnesområden

Fysik
Fysikalisk kemi

Chalmers infrastruktur