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Fluorescence enhancement from single DNA molecules confined in SiO 2 nanochannels

Fredrik Westerlund (Institutionen för fysik (GU) ; Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Fredrik Persson ; A. Kristensen ; Jonas O. Tegenfeldt
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Groningen; Netherlands; 3 October 2010 through 7 October 2010 Vol. 1 (2010), p. 578-580.
[Konferensbidrag, refereegranskat]

We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nanofunnels changes significantly and not monotonically with the width of the funnel, an emission enhancement that is only detected for emitted light polarized parallel to the channel. We explain the enhancement effect as being due to optical phenomena in the channels. The enhancement effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.

Nyckelord: DNA, Fluorescence microscopy, Nanofluidics, Single molecule, Emission enhancement, Emission intensity, Enhancement effects, Fluorescence enhancement, Optical phenomena, Quantitative fluorescence microscopy, Single DNA molecules, Molecules

Denna post skapades 2016-05-12.
CPL Pubid: 236436


Institutioner (Chalmers)

Institutionen för fysik (GU) (GU)
Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)



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