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Probing Microscopic Orientation in Membranes by Linear Dichroism

Sandra Rocha (Institutionen för biologi och bioteknik, Kemisk biologi) ; Maxim Kogan (Institutionen för kemi och kemiteknik, Fysikalisk kemi) ; Tamas Beke-Somfai (Institutionen för kemi och kemiteknik, Fysikalisk kemi) ; Bengt Nordén (Institutionen för kemi och kemiteknik, Fysikalisk kemi)
Langmuir (0743-7463). Vol. 32 (2016), 12, p. 2841-2846.
[Artikel, refereegranskad vetenskaplig]

The cell, membrane is an ordered environment, which anisotropically affects the structure and interactions of all of its molecules. Monitoring membrane orientation at a local level is rather challenging but could reward crucial information on protein conformation and interactions, in the lipid bilayer. We monitored local, lipid ordering. changes upon varying the cholesterol concentration using polarized light spectroscopy and pyrene as a membrane probe., Pyrene, with, a shape intermediate between a disc and a rod, can detect microscopic orientation variations at the level of its site. The global membrane orientation was determined using curcumin, a probe with nonoverlapping absorption relative to that of pyrene. While the macroscopic orientation of a liquid-phase bilayer decreases with increasing cholesterol concentration, the local orientation is improved. Pyrene is,found to be sensitive to-the local effects induced by cholesterol and temperature on the bilayer. Disentangling local and global orientation effects in membranes could provide new insights into functionally significant interactions of membrane proteins.

Nyckelord: flow dichroism, bilayers, curcumin, polarization, spectroscopy, molecules, liposome, spectra, pyrene, lipids, Chemistry, Materials Science

Denna post skapades 2016-04-29. Senast ändrad 2016-07-25.
CPL Pubid: 235628


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