CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases

K. E. H. Frandsen ; T. J. Simmons ; P. Dupree ; J. C. N. Poulsen ; G. R. Hemsworth ; L. Ciano ; E. M. Johnston ; M. Tovborg ; Katja Salomon Johansen (Institutionen för biologi och bioteknik, Industriell bioteknik) ; P. von Freiesleben ; L. Marmuse ; S. Fort ; S. Cottaz ; H. Driguez ; B. Henrissat ; N. Lenfant ; F. Tuna ; A. Baldansuren ; G. J. Davies ; L. Lo Leggio ; P. H. Walton
Nature Chemical Biology (1552-4450). Vol. 12 (2016), 4, p. 298-+.
[Artikel, refereegranskad vetenskaplig]

Lytic polysaccharide monooxygenases (LPMOs) are copper-containing enzymes that oxidatively break down recalcitrant polysaccharides such as cellulose and chitin. Since their discovery, LPMOs have become integral factors in the industrial utilization of biomass, especially in the sustainable generation of cellulosic bioethanol. We report here a structural determination of an LPMO-oligosaccharide complex, yielding detailed insights into the mechanism of action of these enzymes. Using a combination of structure and electron paramagnetic resonance spectroscopy, we reveal the means by which LPMOs interact with saccharide substrates. We further uncover electronic and structural features of the enzyme active site, showing how LPMOs orchestrate the reaction of oxygen with polysaccharide chains.

Denna post skapades 2016-04-21.
CPL Pubid: 234985


Läs direkt!

Länk till annan sajt (kan kräva inloggning)

Institutioner (Chalmers)

Institutionen för biologi och bioteknik, Industriell bioteknik



Chalmers infrastruktur