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Supported lipid bilayer repair mediated by AH peptide

M. C. Kim ; Anders Gunnarsson (Institutionen för fysik, Biologisk fysik (Chalmers)) ; S. R. Tabaei ; Fredrik Höök (Institutionen för fysik, Biologisk fysik (Chalmers)) ; N. J. Cho
Physical Chemistry Chemical Physics (1463-9076). Vol. 18 (2016), 4, p. 3040-3047.
[Artikel, refereegranskad vetenskaplig]

The adsorption and fusion of small unilamellar lipid vesicles on silica-based substrates such as glass is a common method used to fabricate supported lipid bilayers. Successful bilayer formation depends on a number of experimental conditions as well as on the quality of the vesicle preparation. Inevitably, a small fraction of unruptured vesicles always remains in a supported bilayer, and this kind of defect can have devastating influences on the morphological and electrical properties of the supported bilayer when used as a biosensing platform. In this paper, a simple method is reported to improve the completeness of supported bilayers by adding a vesicle rupturing peptide as a final step in the fabrication process. Peptide treatment reduces the fraction of unruptured vesicles to less than 1%, as determined by epifluorescence microscopy and quartz crystal microbalance-dissipation experiments. This step can easily be incorporated into existing procedures for preparing high-quality supported lipid bilayers.

Nyckelord: alpha-helical peptide, quartz-crystal microbalance, phospholipid-bilayers, vesicle adsorption, unilamellar vesicles, osmotic-pressure, in-situ, membranes, rupture, dissipation

Denna post skapades 2016-03-16.
CPL Pubid: 233295


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