CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

Evolution and tinkering: what do a protein kinase, a transcriptional regulator and chromosome segregation/cell division proteins have in common?

Abderahmane Derouiche (Institutionen för biologi och bioteknik, Systembiologi) ; Lei Shi (Institutionen för biologi och bioteknik, Systembiologi) ; Aida Kalantari (Institutionen för biologi och bioteknik, Systembiologi) ; Ivan Mijakovic (Institutionen för biologi och bioteknik, Systembiologi)
Current Genetics (0172-8083). Vol. 62 (2016), 1, p. 67-70.
[Artikel, refereegranskad vetenskaplig]

In this study, we focus on functional interactions among multi-domain proteins which share a common evolutionary origin. The examples we develop are four Bacillus subtilis proteins, which all possess an ATP-binding Walker motif: the bacterial tyrosine kinase (BY-kinase) PtkA, the chromosome segregation protein Soj (ParA), the cell division protein MinD and a transcription regulator SalA. These proteins have arisen via duplication of the ancestral ATP-binding domain, which has undergone fusions with other functional domains in the process of divergent evolution. We point out that these four proteins, despite having very different physiological roles, engage in an unusually high number of binary functional interactions. Namely, MinD attracts Soj and PtkA to the cell pole, and in addition, activates the kinase function of PtkA. SalA also activates the kinase function of PtkA, and it gets phosphorylated by PtkA as well. The consequence of this phosphorylation is the activation of SalA as a transcriptional repressor. We hypothesize that these functional interactions remain preserved during divergent evolution and represent a constraint on the process of evolutionary "tinkering", brought about by fusions of different functional domains.

Nyckelord: Protein phosphorylation, Cell division, DNA-binding domain, Transcriptional regulation, ATPase

Denna post skapades 2016-02-24. Senast ändrad 2016-04-12.
CPL Pubid: 232410


Läs direkt!

Lokal fulltext (fritt tillgänglig)

Länk till annan sajt (kan kräva inloggning)

Institutioner (Chalmers)

Institutionen för biologi och bioteknik, Systembiologi


Biokemi och molekylärbiologi

Chalmers infrastruktur