CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

Formation of reactive aldehydes (MDA, HHE, HNE) during the digestion of cod liver oil: comparison of human and porcine in vitro digestion models

Cecilia Tullberg (Institutionen för biologi och bioteknik, Livsmedelsvetenskap) ; Karin Larsson (Institutionen för biologi och bioteknik, Livsmedelsvetenskap) ; Nils-Gunnar Carlsson (Institutionen för biologi och bioteknik, Livsmedelsvetenskap) ; Irene Comi ; Nathalie Scheers (Institutionen för biologi och bioteknik, Livsmedelsvetenskap) ; Gerd Vegarud ; Ingrid Undeland (Institutionen för biologi och bioteknik, Livsmedelsvetenskap)
Food & Function (2042-6496). Vol. 7 (2016), 3, p. 1401-1412.
[Artikel, refereegranskad vetenskaplig]

In this work, we investigated lipid oxidation of cod liver oil during gastrointestinal (GI) digestion using two types of in vitro digestion models. In the first type of model, we used human GI juices, while we used digestive enzymes and bile from porcine origin in the second type of model. Human and porcine models were matched with respect to factors important for lipolysis, using a standardized digestion protocol. The digests were analysed for reactive oxidation products: malondialdehyde (MDA), 4-hydroxy-trans-2-nonenal (HNE), and 4-hydroxy-trans-2-hexenal (HHE) by liquid chromatography/atmospheric pressure chemical ionization-mass spectrometry (LC/APCI-MS), and for free fatty acids (FFA) obtained during the digestion by gas chromatography-mass spectrometry (GC-MS). The formation of the oxidation products MDA, HHE, and HNE was low during the gastric digestion, however, it increased during the duodenal digestion. The formation of the oxidation products reached higher levels when digestive juices of human origin were used (60 μM of MDA, 0.96 μM of HHE, and 1.6 μM of HNE) compared to when using enzymes and bile of porcine origin (9.8, and 0.36 μM of MDA; 0.16, and 0.026 μM of HHE; 0.23, and 0.005 μM of HNE, respectively, in porcine models I and II). In all models, FFA release was only detected during the intestinal step, and reached up to 31% of total fatty acids (FA). The findings in this work may be of importance when designing oxidation oriented lipid digestion studies.

Nyckelord: Lipid oxidation, in vitro digestion, cod liver oil

Den här publikationen ingår i följande styrkeområden:

Läs mer om Chalmers styrkeområden  

Denna post skapades 2016-02-24. Senast ändrad 2016-08-09.
CPL Pubid: 232378


Läs direkt!

Lokal fulltext (fritt tillgänglig)

Länk till annan sajt (kan kräva inloggning)

Institutioner (Chalmers)

Institutionen för biologi och bioteknik, Livsmedelsvetenskap



Chalmers infrastruktur