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The Fun30 Chromatin Remodeler Fft3 Controls Nuclear Organization and Chromatin Structure of Insulators and Subtelomeres in Fission Yeast

Babett Steglich ; Annelie Strålfors ; Olga Khorosjutina ; Jenna Persson ; Agata Smialowska (Institutionen för kemi och kemiteknik) ; Jean Paul Javerzat ; Karl Ekwall
PLoS Genetics (1553-7390). Vol. 11 (2015), 3, p. e1005101.
[Artikel, refereegranskad vetenskaplig]

In eukaryotic cells, local chromatin structure and chromatin organization in the nucleus both influence transcriptional regulation. At the local level, the Fun30 chromatin remodeler Fft3 is essential for maintaining proper chromatin structure at centromeres and subtelomeres in fission yeast. Using genome-wide mapping and live cell imaging, we show that this role is linked to controlling nuclear organization of its targets. In fft3? cells, subtelomeres lose their association with the LEM domain protein Man1 at the nuclear periphery and move to the interior of the nucleus. Furthermore, genes in these domains are upregulated and active chromatin marks increase. Fft3 is also enriched at retrotransposon-derived long terminal repeat (LTR) elements and at tRNA genes. In cells lacking Fft3, these sites lose their peripheral positioning and show reduced nucleosome occupancy. We propose that Fft3 has a global role in mediating association between specific chromatin domains and the nuclear envelope.

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Denna post skapades 2016-01-04. Senast ändrad 2016-03-10.
CPL Pubid: 229514


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