CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

Resistance Mutations in gyrA and parC are Common in Escherichia Communities of both Fluoroquinolone-Polluted and Uncontaminated Aquatic Environments

Anna Johnning (Institutionen för matematiska vetenskaper, matematisk statistik ; Institutionen för biomedicin, avdelningen för infektionssjukdomar) ; Erik Kristiansson (Institutionen för matematiska vetenskaper, matematisk statistik) ; Jerker Fick ; Birgitta Weijdegård ; D. G. Joakim Larsson
Frontiers in Microbiology (1664-302X). Vol. 6 (2015), p. 1355.
[Artikel, refereegranskad vetenskaplig]

Alterations in the target proteins of fluoroquinolones, especially in GyrA and ParC, are known to cause resistance. Here, we investigated environmental Escherichia communities to explore the possible link between the abundance of mutations, and the exposure to fluoroquinolones. Sediment samples were collected from a relatively pristine lake, up and downstream from a sewage treatment plant, and from several industrially polluted sites. The quinolone resistance-determining regions of gyrA and parC were analyzed using amplicon sequencing of metagenomic DNA. Five non-synonymous substitutions were present in all samples, and all of these mutations have been previously linked to fluoroquinolone resistance in Escherichia coli. In GyrA, substitutions S83L and D87N were on average detected at frequencies of 86 and 32%, respectively, and 31% of all amplicons encoded both substitutions. In ParC, substitutions S80I, E84G, and E84V were detected in 42, 0.9, and 6.0% of the amplicons, respectively, and 6.5% encoded double substitutions. There was no significant correlation between the level of fluoroquinolone pollution and the relative abundance of resistance mutations, with the exception of the most polluted site, which showed the highest abundance of said substitutions in both genes. Our results demonstrate that resistance mutations can be common in environmental Escherichia, even in the absence of a fluoroquinolone selective pressure.

Nyckelord: Fluoroquinolones, pharmaceutical pollution, antibiotic resistance



Den här publikationen ingår i följande styrkeområden:

Läs mer om Chalmers styrkeområden  

Denna post skapades 2015-12-15. Senast ändrad 2016-11-07.
CPL Pubid: 228285

 

Läs direkt!

Lokal fulltext (fritt tillgänglig)

Länk till annan sajt (kan kräva inloggning)


Institutioner (Chalmers)

Institutionen för matematiska vetenskaper, matematisk statistik (2005-2016)
Institutionen för biomedicin, avdelningen för infektionssjukdomar (GU)

Ämnesområden

Livsvetenskaper
Mikrobiologi
Bioinformatik och systembiologi

Chalmers infrastruktur