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Electrochemical Measurements of Optogenetically Stimulated Quantal Amine Release from Single Nerve Cell Varicosities in Drosophila Larvae

Soodabeh Majdi (Institutionen för kemi och kemiteknik, Analytisk kemi) ; E Carina Berglund ; Johan Dunevall (Institutionen för kemi och kemiteknik, Analytisk kemi) ; A. I. Oleinick ; C. Amatore ; D. E. Krantz ; Andrew G Ewing (Institutionen för kemi och molekylärbiologi ; Institutionen för kemi och kemiteknik, Analytisk kemi)
Angewandte Chemie International Edition (1433-7851). Vol. 54 (2015), 46, p. 13609-13612.
[Artikel, refereegranskad vetenskaplig]

The nerve terminals found in the body wall of Drosophila melanogaster larvae are readily accessible to experimental manipulation. We used the light-activated ion channel, channelrhodopsin-2, which is expressed by genetic manipulation in TypeII varicosities to study octopamine release in Drosophila. We report the development of a method to measure neurotransmitter release from exocytosis events at individual varicosities in the Drosophila larval system by amperometry. A microelectrode was placed in a region of the muscle containing a varicosity and held at a potential sufficient to oxidize octopamine and the terminal stimulated by blue light. Optical stimulation of TypeII boutons evokes exocytosis of octopamine, which is detected through oxidization at the electrode surface. We observe 22700 +/- 4200 molecules of octopamine released per vesicle. This system provides a genetically accessible platform to study the regulation of amine release at an intact synapse.

Nyckelord: amperometry, channelrhodopsin, Drosophila larvae, octopamine, varicosity



Denna post skapades 2015-12-14. Senast ändrad 2017-01-27.
CPL Pubid: 228159

 

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Institutioner (Chalmers)

Institutionen för kemi och kemiteknik, Analytisk kemi
Institutionen för kemi och molekylärbiologi (GU)

Ämnesområden

Biologiska vetenskaper
Cellbiologi

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