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Catalase improves saccharification of lignocellulose by reducing lytic polysaccharide monooxygenase-associated enzyme inactivation

Katja Salomon Johansen (Institutionen för biologi och bioteknik, Industriell bioteknik) ; Brian R. Scott ; Hong Zhi Huang ; Jesper Frickman ; Rune Halvorsen
Biotechnology Letters (0141-5492). Vol. 38 (2016), 3, p. 425-434.
[Artikel, refereegranskad vetenskaplig]

Objectives Efficient enzymatic saccharification of plant cell wall material is key to industrial processing of agricultural and forestry waste such as straw and wood chips into fuels and chemicals. Results Saccharification assays were performed on steam-pretreated wheat straw under ambient and O2-deprived environments and in the absence and presence of a lytic polysaccharide monooxygenase (LPMO) and catalase. A kinetic model was used to calculate catalytic rate and first-order inactivation rate constants of the cellulases from reaction progress curves. The addition of a LPMO significantly (P < 0.01, Student’s T test) enhanced the rate of glucose release from 2.8 to 6.9 h−1 under ambient O2 conditions. However, this also significantly (P < 0.01, Student’s T test) increased the rate of inactivation of the enzyme mixture, thereby reducing the performance half-life from 65 to 35 h. Decreasing O2 levels or, strikingly, the addition of catalase significantly reduced (P < 0.01, Student’s T test) enzyme inactivation and, as a consequence, higher efficiency of the cellulolytic enzyme cocktail was achieved. Conclusion Oxidative inactivation of commercial cellulase mixtures is a significant factor influencing the overall saccharification efficiency and the addition of catalase can be used to protect these mixtures from inactivation.

Nyckelord: Catalase, Cellulase, Fenton chemistry, Kinetic modelling, Lytic polysaccharide monooxygenase, Pretreated wheat straw, Reactive oxygen species



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Denna post skapades 2015-12-07. Senast ändrad 2016-03-30.
CPL Pubid: 227494

 

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Institutioner (Chalmers)

Institutionen för biologi och bioteknik, Industriell bioteknik

Ämnesområden

Energi
Kemi
Biologiska vetenskaper
Kemiteknik

Chalmers infrastruktur