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The gut microbiota modulates host amino acid and glutathione metabolism in mice

Adil Mardinoglu (Institutionen för biologi och bioteknik, Systembiologi) ; Saeed Shoaie (Institutionen för biologi och bioteknik, Systembiologi) ; Mattias Bergentall ; Pouyan Ghaffari Nouran (Institutionen för biologi och bioteknik, Systembiologi) ; C. Zhang ; Erik Larsson ; Fredrik Bäckhed ; Jens B. Nielsen (Institutionen för biologi och bioteknik, Systembiologi)
Molecular Systems Biology (1744-4292). Vol. 11 (2015), 10, p. Article Number: 834.
[Artikel, refereegranskad vetenskaplig]

The gut microbiota has been proposed as an environmental factor that promotes the progression of metabolic diseases. Here, we investigated how the gut microbiota modulates the global metabolic differences in duodenum, jejunum, ileum, colon, liver, and two white adipose tissue depots obtained from conventionally raised (CONV-R) and germ-free (GF) mice using gene expression data and tissue-specific genome-scale metabolic models (GEMs). We created a generic mouse metabolic reaction (MMR) GEM, reconstructed 28 tissue-specific GEMs based on proteomics data, and manually curated GEMs for small intestine, colon, liver, and adipose tissues. We used these functional models to determine the global metabolic differences between CONV-R and GF mice. Based on gene expression data, we found that the gut microbiota affects the host amino acid (AA) metabolism, which leads to modifications in glutathione metabolism. To validate our predictions, we measured the level of AAs and N-acetylated AAs in the hepatic portal vein of CONV-R and GF mice. Finally, we simulated the metabolic differences between the small intestine of the CONV-R and GF mice accounting for the content of the diet and relative gene expression differences. Our analyses revealed that the gut microbiota influences host amino acid and glutathione metabolism in mice.

Nyckelord: genome-scale metabolic models, germ-free mice, glutathione metabolism, metabolomics, transcriptomics



Denna post skapades 2015-11-30. Senast ändrad 2016-01-29.
CPL Pubid: 226625

 

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Institutioner (Chalmers)

Institutionen för biologi och bioteknik, Systembiologi
Wallenberglaboratoriet (GU)
Institutionen för medicin, avdelningen för molekylär och klinisk medicin (GU)

Ämnesområden

Biokemi
Cell- och molekylärbiologi

Chalmers infrastruktur

 


Projekt

Denna publikation är ett resultat av följande projekt:


Metagenomics in Cardiometabolic Diseases (METACARDIS) (EC/FP7/305312)