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Neuronal Networks on Nanocellulose Scaffolds

M. Jonsson ; Christian Brackmann (Institutionen för biologi och bioteknik) ; Maja Puchades (Institutionen för biologi och bioteknik) ; K. Brattås ; Andrew G Ewing (Institutionen för kemi och kemiteknik, Analytisk kemi) ; Paul Gatenholm (Institutionen för kemi och kemiteknik, Polymerteknologi) ; Annika Enejder (Institutionen för biologi och bioteknik, Kemisk biologi)
Tissue Engineering. Part C, Methods (1937-3384). Vol. 21 (2015), 11, p. 1162-1170.
[Artikel, refereegranskad vetenskaplig]

Proliferation, integration, and neurite extension of PC12 cells, a widely used culture model for cholinergic neurons, were studied in nanocellulose scaffolds biosynthesized by Gluconacetobacter xylinus to allow a three-dimensional (3D) extension of neurites better mimicking neuronal networks in tissue. The interaction with control scaffolds was compared with cationized nanocellulose (trimethyl ammonium betahydroxy propyl [TMAHP] cellulose) to investigate the impact of surface charges on the cell interaction mechanisms. Furthermore, coatings with extracellular matrix proteins (collagen, fibronectin, and laminin) were investigated to determine the importance of integrin-mediated cell attachment. Cell proliferation was evaluated by a cellular proliferation assay, while cell integration and neurite propagation were studied by simultaneous label-free Coherent anti-Stokes Raman Scattering and second harmonic generation microscopy, providing 3D images of PC12 cells and arrangement of nanocellulose fibrils, respectively. Cell attachment and proliferation were enhanced by TMAHP modification, but not by protein coating. Protein coating instead promoted active interaction between the cells and the scaffold, hence lateral cell migration and integration. Irrespective of surface modification, deepest cell integration measured was one to two cell layers, whereas neurites have a capacity to integrate deeper than the cell bodies in the scaffold due to their fine dimensions and amoeba-like migration pattern. Neurites with lengths of >50 μm were observed, successfully connecting individual cells and cell clusters. In conclusion, TMAHP-modified nanocellulose scaffolds promote initial cellular scaffold adhesion, which combined with additional cell-scaffold treatments enables further formation of 3D neuronal networks.

Denna post skapades 2015-11-19. Senast ändrad 2016-07-14.
CPL Pubid: 225998


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