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The Orientation of Nanoscale Apatite Platelets in Relation to Osteoblastic-Osteocyte Lacunae on Trabecular Bone Surface.

Furqan A. Shah ; Ezio Zanghellini (Institutionen för teknisk fysik, Kondenserade materiens fysik) ; Aleksandar Matic (Institutionen för teknisk fysik, Kondenserade materiens fysik) ; Peter Thomsen ; Anders Palmquist
Calcified Tissue International (0171-967X). Vol. 98 (2016), 2, p. 193-205.
[Artikel, refereegranskad vetenskaplig]

The orientation of nanoscale mineral platelets was quantitatively evaluated in relation to the shape of lacunae associated with partially embedded osteocytes (osteoblastic-osteocytes) on the surface of deproteinised trabecular bone of adult sheep. By scanning electron microscopy and image analysis, the mean orientation of mineral platelets at the osteoblastic-osteocyte lacuna (Ot.Lc) floor was found to be 19° ± 14° in the tibia and 20° ± 14° in the femur. Further, the mineral platelets showed a high degree of directional coherency: 37 ± 7 % in the tibia and 38 ± 9 % in the femur. The majority of Ot.Lc in the tibia (69.37 %) and the femur (74.77 %) exhibited a mean orientation of mineral platelets between 0° and 25°, with the largest fraction within a 15°-20° range, 17.12 and 19.8 % in the tibia and femur, respectively. Energy dispersive X-ray spectroscopy and Raman spectroscopy were used to characterise the features observed on the anorganic bone surface. The Ca/P (atomic %) ratio was 1.69 ± 0.1 within the Ot.Lc and 1.68 ± 0.1 externally. Raman spectra of NaOCl-treated bone showed peaks associated with carbonated apatite: ν1, ν2 and ν4 PO4 (3-), and ν1 CO3 (2-), while the collagen amide bands were greatly reduced in intensity compared to untreated bone. The apatite-to-collagen ratio increased considerably after deproteinisation; however, the mineral crystallinity and the carbonate-to-phosphate ratios were unaffected. The ~19°-20° orientation of mineral platelets in at the Ot.Lc floor may be attributable to a gradual rotation of osteoblasts in successive layers relative to the underlying surface, giving rise to the twisted plywood-like pattern of lamellar bone.

Nyckelord: Bone, Biomineralisation, Osteoblastic-osteocyte, Lacuna, Trabecular bone, Electron microscopy, Raman spectroscopy

Denna post skapades 2015-11-19. Senast ändrad 2016-02-24.
CPL Pubid: 225963


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Institutioner (Chalmers)

Institutionen för kliniska vetenskaper, sektionen för anestesi, biomaterial och ortopedi, Avdelningen för biomaterialvetenskap (GU)
Institutionen för teknisk fysik, Kondenserade materiens fysik (1900-2015)



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