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Micro-droplet based directed evolution outperforms conventional laboratory evolution

S.L. Sjostrom ; Mingtao Huang (Institutionen för biologi och bioteknik, Systembiologi) ; Jens B. Nielsen (Institutionen för biologi och bioteknik, Systembiologi) ; H.N. Joensson ; H. Andersson Svahn
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014; United States; 26-30 October 2014 p. 169-171. (2015)
[Konferensbidrag, refereegranskat]

We present droplet adaptive laboratory evolution (DrALE), a directed evolution method used to improve industrial enzyme producing microorganisms for e.g. feedstock digestion. DrALE is based linking a desired phenotype to growth rate allowing only desired cells to proliferate. Single cells are confined in microfluidic droplets to prevent the phenotype, e.g. secreted enzymes, from leaking between cells. The method was benchmarked against and found to significantly outperform conventional adaptive laboratory evolution (ALE) in enriching enzyme producing cells. It was furthermore applied to enrich a whole-genome mutated library of yeast cells for α-amylase activity.

Nyckelord: Cell factories; Directed evolution; Droplet microfluidics; Enzymes; High throughput

Denna post skapades 2015-09-30. Senast ändrad 2016-06-30.
CPL Pubid: 223464


Institutioner (Chalmers)

Institutionen för biologi och bioteknik, Systembiologi


Bioinformatik och systembiologi

Chalmers infrastruktur