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Genome-wide expression analyses of the stationary phase model of ageing in yeast

Kwanjeera Wanichthanarak (Institutionen för biologi och bioteknik, Systembiologi) ; Nutvadee Wongtosrad (Institutionen för matematiska vetenskaper) ; Dina Petranovic (Institutionen för biologi och bioteknik, Systembiologi)
Mechanisms of Ageing and Development (0047-6374). Vol. 149 (2015), p. 65-74.
[Artikel, refereegranskad vetenskaplig]

Ageing processes involved in replicative lifespan (RLS) and chronological lifespan (CLS) have been found to be conserved among many organisms, including in unicellular Eukarya such as yeast Saccharomyces cerevisiae. Here we performed an integrated approach of genome wide expression profiles of yeast at different time points, during growth and starvation. The aim of the study was to identify transcriptional changes in those conditions by using several different computational analyses in order to propose transcription factors, biological networks and metabolic pathways that seem to be relevant during the process of chronological ageing in yeast. Specifically, we performed differential gene expression analysis, gene-set enrichment analysis and network-based analysis, and we identified pathways affected in the stationary phase and specific transcription factors driving transcriptional adaptations. The results indicate signal propagation from G protein-coupled receptors through signaling pathway components and other stress and nutrient-induced transcription factors resulting in adaptation of yeast cells to the lack of nutrients by activating metabolism associated with aerobic metabolism of carbon sources such as ethanol, glycerol and fatty acids. In addition, we found STE12, XBP1 and TOS8 as highly connected nodes in the subnetworks of ageing yeast.

Nyckelord: Gene expression analyses , Integrated analyses , Nutritional starvation , Yeast chronological lifespan

Denna post skapades 2015-07-21.
CPL Pubid: 219874


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Institutionen för biologi och bioteknik, Systembiologi
Institutionen för matematiska vetenskaperInstitutionen för matematiska vetenskaper (GU)


Biokemi och molekylärbiologi

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