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Advances in yeast genome engineering

Florian David (Institutionen för biologi och bioteknik, Systembiologi) ; Verena Siewers (Institutionen för biologi och bioteknik, Systembiologi)
Fems Yeast Research (1567-1356). Vol. 15 (2015), 1,
[Artikel, refereegranskad vetenskaplig]

Genome engineering based on homologous recombination has been applied to yeast for many years. However, the growing importance of yeast as a cell factory in metabolic engineering and chassis in synthetic biology demands methods for fast and efficient introduction of multiple targeted changes such as gene knockouts and introduction of multistep metabolic pathways. In this review, we summarize recent improvements of existing genome engineering methods, the development of novel techniques, for example for advanced genome redesign and evolution, and the importance of endonucleases as genome engineering tools.

Nyckelord: Genome editing, Sc2.0, endonucleases, adaptive laboratory evolution



Denna post skapades 2015-07-20.
CPL Pubid: 219845

 

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Institutioner (Chalmers)

Institutionen för biologi och bioteknik, Systembiologi

Ämnesområden

Bioinformatik och systembiologi

Chalmers infrastruktur