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Enhanced amino acid utilization sustains growth of cells lacking Snf1/AMPK

R. Nicastro ; F. Tripodi ; C. Guzzi ; V. Reghellin ; Sakda Khoomrung (Institutionen för biologi och bioteknik, Systembiologi) ; C. Capusoni ; C. Compagno ; C. Airoldi ; Jens B. Nielsen (Institutionen för biologi och bioteknik, Systembiologi) ; L. Alberghina ; P. Coccetti
Biochimica Et Biophysica Acta-Molecular Cell Research (0167-4889). Vol. 1853 (2015), 7, p. 1615-1625.
[Artikel, refereegranskad vetenskaplig]

The metabolism of proliferating cells shows common features even in evolutionary distant organisms such as mammals and yeasts, for example the requirement for anabolic processes under tight control of signaling pathways. Analysis of the rewiring of metabolism, which occurs following the dysregulation of signaling pathways, provides new knowledge about the mechanisms underlying cell proliferation. The key energy regulator in yeast Snf1 and its mammalian ortholog AMPK have earlier been shown to have similar functions at glucose limited conditions and here we show that they also have analogies when grown with glucose excess. We show that loss of Snf1 in cells growing in 2% glucose induces an extensive transcriptional reprogramming, enhances glycolytic activity, fatty add accumulation and reliance on amino acid utilization for growth. Strikingly, we demonstrate that Snf1/AMPK-deficient cells remodel their metabolism fueling mitochondria and show glucose and amino acids addiction, a typical hallmark of cancer cells.

Nyckelord: Saccharomyces cerevisiae, Glucose, Budding yeast, Metabolism, Respiration, Gene chip

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Denna post skapades 2015-06-29. Senast ändrad 2017-01-17.
CPL Pubid: 219000


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Institutionen för biologi och bioteknik, Systembiologi


Cell- och molekylärbiologi

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