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Detection of peptide-based nanoparticles in blood plasma by ELISA

G.H. Bode ; K.E. Pickl ; M. Sanchez-Purrà ; B. Albaiges ; S. Borrós ; A.J.G. Pötgens ; C. Schmitz ; F.M. Sinner ; M. Losen ; H.W.M. Steinbusch ; H.G. Frank ; P. Martinez-Martinez ; K. Wagemann ; H.A. Klok ; R.E. Unger ; T. Pieber ; A. Cesàro ; J. Engbersen ; Bengt Kasemo (Institutionen för teknisk fysik, Kemisk fysik) ; M. Moeller ; R. Korenstein ; C. Grandfils ; A. Bernkop-Schnuerch ; C. Kiparissides ; S. Slomkowski ; P. Venturini ; C.M. Paleos ; B. Podobnik ; P. Borm ; E.C.A. Van Winden ; J. Groll ; B. Zassler ; G. Gregoriadis ; P.K. Nielsen ; A. Elouahabi
PLoS ONE (1932-6203). Vol. 10 (2015), 5, p. Art. no. e0126136.
[Artikel, refereegranskad vetenskaplig]

Aims: The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma. Materials & Methods: A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology. Results: The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl meth-acrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS. Conclusions: We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.



Denna post skapades 2015-06-24. Senast ändrad 2015-12-16.
CPL Pubid: 218761

 

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Institutioner (Chalmers)

Institutionen för teknisk fysik, Kemisk fysik (1900-2015)

Ämnesområden

Analytisk kemi
Medicinsk bioteknologi (med inriktning mot cellbiologi)

Chalmers infrastruktur