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Spatial resolution of exocytosis across a single cell by a microwell-based individually addressable thin film ultra-microelectrode array

Jun Wang (Institutionen för kemi och molekylärbiologi ; Institutionen för kemi- och bioteknik, Analytisk kemi) ; Raphaël Trouillon ; Johan Dunevall (Institutionen för kemi- och bioteknik, Analytisk kemi) ; Andrew G Ewing (Institutionen för kemi och molekylärbiologi ; Institutionen för kemi- och bioteknik, Analytisk kemi)
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013 Vol. 1 (2013), p. 392-394.
[Konferensbidrag, refereegranskat]

We report the fabrication and characterization of micro well-based individually addressable ultra-microelectrode arrays (MEAs) and their application to spatially and temporally resolved detection of neurotransmitter release across a single pheochromocytoma (PC 12) cell. The MEAs consist of sixteen 4-μm-width square microelectrodes, or twenty-five 3-μm-width square microelectrodes, or thirty-six 2-jim-width square microelectrodes. Each MEA is tightly defined in a 30×30 μm square area, which is further encased inside a 40×40 μm SU-8 microwell. We demonstrate the excellent stability and reproducibility of these microelectrodes by using cyclic voltammetry and we have performed recording of spatially resolution of single cell exocytosis with multiple ultra-microelectrodes in 2-μm resolution.

Nyckelord: Microwell-based ultra-microelectrode array; Single cell exocytosis; Spatial and temporal resolution


Länk till proceedings innehållsförteckning: http://toc.proceedings.com/22480webtoc.pdf



Denna post skapades 2015-05-06. Senast ändrad 2016-11-08.
CPL Pubid: 216669

 

Institutioner (Chalmers)

Institutionen för kemi och molekylärbiologi (GU)
Institutionen för kemi- och bioteknik, Analytisk kemi (2006-2014)

Ämnesområden

Kemiteknik

Chalmers infrastruktur