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Novel bilayer bacterial nanocellulose scaffold supports neocartilage formation in vitro and in vivo

Héctor Martínez Ávila (Institutionen för kemi och kemiteknik, Polymerteknologi) ; E.M. Feldmann ; M.M. Pleumeekers ; L. Nimeskern ; W. Kuo ; W.C. De Jong ; S. Schwarz ; R. Müller ; J.A.A. Hendriks ; N. Rotter ; G.J.V.M. Van Osch ; K.S. Stok ; Paul Gatenholm (Institutionen för kemi och kemiteknik, Polymerteknologi)
Biomaterials (0142-9612). Vol. 44 (2015), p. 122-133.
[Artikel, refereegranskad vetenskaplig]

Tissue engineering provides a promising alternative therapy to the complex surgical reconstruction of auricular cartilage by using ear-shaped autologous costal cartilage. Bacterial nanocellulose (BNC) is proposed as a promising scaffold material for auricular cartilage reconstruction, as it exhibits excellent biocompatibility and secures tissue integration. Thus, this study evaluates a novel bilayer BNC scaffold for auricular cartilage tissue engineering. Bilayer BNC scaffolds, composed of a dense nanocellulose layer joined with a macroporous composite layer of nanocellulose and alginate, were seeded with human nasoseptal chondrocytes (NC) and cultured invitro for up to 6 weeks. To scale up for clinical translation, bilayer BNC scaffolds were seeded with a low number of freshly isolated (uncultured) human NCs combined with freshly isolated human mononuclear cells (MNC) from bone marrow in alginate and subcutaneously implanted in nude mice for 8 weeks. 3D morphometric analysis showed that bilayer BNC scaffolds have a porosity of 75% and mean pore size of 50±25μm. Furthermore, endotoxin analysis and invitro cytotoxicity testing revealed that the produced bilayer BNC scaffolds were non-pyrogenic (0.15±0.09EU/ml) and non-cytotoxic (cell viability: 97.8±4.7%). This study demonstrates that bilayer BNC scaffolds offer a good mechanical stability and maintain a structural integrity while providing a porous architecture that supports cell ingrowth. Moreover, bilayer BNC scaffolds provide a suitable environment for culture-expanded NCs as well as a combination of freshly isolated NCs and MNCs to form cartilage invitro and invivo as demonstrated by immunohistochemistry, biochemical and biomechanical analyses.

Nyckelord: Tissue engineering, Ear cartilage, Neo-cartilage, Bacterial cellulose, Nasoseptal chondrocytes, Mononuclear cells

Denna post skapades 2015-03-12. Senast ändrad 2015-03-26.
CPL Pubid: 213719


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