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ToF-SIMS imaging of lipids and lipid related compounds in Drosophila brain

Nhu TN Phan ; John S. Fletcher ; Peter Sjövall ; Andrew G Ewing (Institutionen för kemi och molekylärbiologi ; Institutionen för kemi- och bioteknik, Analytisk kemi)
Surface and Interface Analysis: 19th International Conference on Secondary Ion Mass Spectrometry (SIMS) (0142-2421). Vol. 46 (2014), S1, p. 123-126.
[Konferensbidrag, refereegranskat]

Drosophila melanogaster (fruit fly) has a relatively simple nervous system but possesses high order brain functions similar to humans. Therefore, it has been used as a common model system in biological studies, particularly drug addiction. Here, the spatial distribution of biomolecules in the brain of the fly was studied using time-of-flight SIMS. Fly brains were analyzed frozen to prevent molecular redistribution prior to analysis. Different molecules were found to distribute differently in the tissue, particularly the eye pigments, diacylglycerides, and phospholipids, and this is expected to be driven by their biological functions in the brain. Correlations in the localization of these molecules were also observed using principal components analysis of image data, and this was used to identify peaks for further analysis. Furthermore, consecutive analyses following 10keV Ar-2500(+) sputtering showed that different biomolecules respond differently to Ar-2500(+) sputtering. Significant changes in signal intensities between consecutive analyses were observed for high mass molecules including lipids.

Nyckelord: SIMS, imaging mass spectrometry, Drosophila, lipids



Denna post skapades 2015-01-08. Senast ändrad 2016-08-17.
CPL Pubid: 210189

 

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Institutioner (Chalmers)

Institutionen för kemi och molekylärbiologi (GU)
Institutionen för kemi- och bioteknik, Analytisk kemi (2006-2014)

Ämnesområden

Fysikalisk kemi

Chalmers infrastruktur