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DNA-Binding Properties of Amidine-Substituted Spiropyran Photoswitches

Martin Hammarson (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Jesper Nilsson (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Shiming Li (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Per Lincoln (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Joakim Andréasson (Institutionen för kemi- och bioteknik, Fysikalisk kemi)
Chemistry - A European Journal (0947-6539). Vol. 20 (2014), 48, p. 15855-15862.
[Artikel, refereegranskad vetenskaplig]

Two amidine-substituted spiropyran derivatives have been characterized with respect to the DNA-binding properties over a broad pH interval. The two derivatives differ in the number of positive charges. By varying the pH, the protonation state of the derivatives is also changed, allowing for additional variations in the charge distribution. We show that the closed spiro isomer does not bind for either of the two derivatives, whereas the open merocyanine forms bind both in the protonated and in the nonprotonated state, but with dramatically different binding constants. Flow-oriented linear dichroism (LD) measurements also show that there are differences in the binding modes between the various forms. We rationalize these differences in terms of structure and charge distribution.

Nyckelord: DNA, DNA-binding, photochromism, photoswitches, spiropyrans

Denna post skapades 2015-01-05. Senast ändrad 2015-01-27.
CPL Pubid: 209535


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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)



Chalmers infrastruktur



Denna publikation är ett resultat av följande projekt:

Photochromic systems for solid state molecular electronic devices and light-activated cancer drugs (PHOTOCHROMES) (EC/FP7/203952)