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Production of 2-butanol through meso-2,3-butanediol consumption in lactic acid bacteria

Payam Ghiaci (Institutionen för kemi- och bioteknik, Systembiologi) ; Francisca Lameiras (Institutionen för kemi- och bioteknik, Systembiologi) ; Joakim Norbeck (Institutionen för kemi- och bioteknik, Systembiologi) ; Christer Larsson (Institutionen för kemi- och bioteknik, Systembiologi)
FEMS Microbiology Letters (0378-1097). Vol. 360 (2014), 1, p. 70-75.
[Artikel, refereegranskad vetenskaplig]

2-Butanol has been an issue of industries in many areas, for example, biofuel production (as an advanced alternate fuel), fermented beverages, and food (as taste-altering component). Thus, its source of production, the biological pathway, and the enzymes involved are of high interest. In this study, 42 different isolates of lactic acid bacteria from nine different species were screened for their capability to consume meso-2,3-butanediol and produce 2-butanol. Lactobacillus brevis was the only species that showed any production of 2-butanol. Five of ten tested isolates of L.brevis were able to convert meso-2,3-butanediol to 2-butanol in a synthetic medium (SM2). However, none of them showed the same capability in a complex medium such as MRS indicating that the ability to produce 2-butanol is subject to some kind of repression mechanism. Furthermore, by evaluating the performance of the enzymes required to convert meso-2,3-butanediol to 2-butanol, that is, the secondary alcohol dehydrogenase and the diol dehydratase, it was shown that the latter needed the presence of a substrate to be expressed.

Nyckelord: lactic acid bacteria, biofuel, microbial biochemistry



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Denna post skapades 2014-12-16. Senast ändrad 2014-12-16.
CPL Pubid: 208236

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Systembiologi (2008-2014)

Ämnesområden

Energi
Mikrobiologi

Chalmers infrastruktur