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Clustered DNA damage on subcellular level: effect of scavengers

Katerina Pachnerova Brabcova (Institutionen för teknisk fysik, Nukleär teknik) ; Lembit Sihver (Institutionen för teknisk fysik, Nukleär teknik) ; N. Yasuda ; Y. Matuo ; V. Stepan ; M. Davidkova
Radiation and Environmental Biophysics (0301-634X). Vol. 53 (2014), 4, p. 705-712.
[Artikel, refereegranskad vetenskaplig]

Clustered DNA damages are induced by ionizing radiation, particularly of high linear energy transfer (LET). Compared to isolated DNA damage sites, their biological effects can be more severe. We investigated a clustered DNA damage induced by high LET radiation (C 290 MeV u(-1) and Fe 500 MeV u(-1)) in pBR322 plasmid DNA. The plasmid is dissolved in pure water or in aqueous solution of one of the three scavengers (coumarin-3-carboxylic acid, dimethylsulfoxide, and glycylglycine). The yield of double strand breaks (DSB) induced in the DNA plasmid-scavenger system by heavy ion radiation was found to decrease with increasing scavenging capacity due to reaction with hydroxyl radical, linearly with high correlation coefficients. The yield of non-DSB clusters was found to occur twice as much as the DSB. Their decrease with increasing scavenging capacity had lower linear correlation coefficients. This indicates that the yield of non-DSB clusters depends on more factors, which are likely connected to the chemical properties of individual scavengers.

Nyckelord: Clustered damage, Indirect effects, Heavy ion, Plasmid in liquid water, Scavenger

Denna post skapades 2014-12-08.
CPL Pubid: 207621


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Institutioner (Chalmers)

Institutionen för teknisk fysik, Nukleär teknik (2006-2015)


Teknisk fysik

Chalmers infrastruktur