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Dietary polyunsaturated fatty acids increase survival and decrease bacterial load during septic S. aureus infection, and improve neutrophil function in mice

Sara L Svahn ; Louise Grahnemo ; Vilborg Palsdottir ; Intawat Nookaew (Institutionen för biologi och bioteknik, Systembiologi) ; Karl Wendt ; Britt G. Gabrielsson (Institutionen för biologi och bioteknik, Livsmedelsvetenskap) ; Erik Scheele ; Anna Benrick ; Niklas Andersson ; Staffan Nilsson (Institutionen för matematiska vetenskaper, matematisk statistik) ; Maria E Johansson ; John-Olov Jansson
Infection and Immunity (0019-9567). Vol. 83 (2015), 2, p. 514-21.
[Artikel, refereegranskad vetenskaplig]

Severe infection, including sepsis, is an increasing clinical problem that causes prolonged morbidity and substantial mortality. At present, antibiotics are essentially the only pharmacological treatment for sepsis. The incidence of resistance to antibiotics is increasing and it is therefore critical to find new therapies for sepsis. Staphylococcus aureus (S. aureus) is a major cause of septic mortality. Neutrophils play an important role in the defense against bacterial infections. We have shown that a diet with high levels of dietary saturated fatty acids decreases survival in septic mice, but the mechanisms behind remain elusive. The aim of the present study was to investigate how the differences in dietary fat composition affect survival and bacterial load after experimental septic infection and neutrophil function in uninfected mice. We found that, after S. aureus infection, mice fed polyunsaturated high fat diet (HFD/P) for 8 weeks had increased survival and decreased bacterial load during sepsis compared with mice fed saturated high fat diet (HFD/S), and similar to that of mice fed low fat diet (LFD). Uninfected mice fed HFD/P had increased frequency of neutrophils in bone marrow compared with mice fed HFD/S. In addition, mice fed HFD/P had a higher frequency of neutrophils recruited to the site of inflammation in response to peritoneal injection of thioglycollate compared with HFD/S. Differences between the proportion of dietary protein and carbohydrate did not affect septic survival at all. In conclusion, polyunsaturated dietary fat increased both survival and efficiency of bacterial clearance during septic S. aureus infection. Moreover, this diet increased the frequency and chemotaxis of neutrophils, key components of the immune response to S. aureus infections.

Nyckelord: S. aureus, septic infection, neutrophils, dietary fat, polyunsaturated high fat diet, saturated high fat diet



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Denna post skapades 2014-12-04. Senast ändrad 2016-06-29.
CPL Pubid: 207293

 

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Institutioner (Chalmers)

Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi (GU)
Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning (GU)
Institutionen för biologi och bioteknik, Systembiologi
Institutionen för biologi och bioteknik, Livsmedelsvetenskap
Institutionen för matematiska vetenskaper, matematisk statistik (2005-2016)

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Livsvetenskaper
Hållbar utveckling
Fysiologi
Bakteriologi
Immunbiologi
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