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Kinetic modeling of multi-feed simultaneous saccharification and co-fermentation of pretreated birch to ethanol

Ruifei Wang (Institutionen för kemi- och bioteknik, Industriell Bioteknik ) ; Rakesh Koppram (Institutionen för kemi- och bioteknik, Industriell Bioteknik ) ; Lisbeth Olsson (Institutionen för kemi- och bioteknik, Industriell Bioteknik ) ; Carl Johan Franzén (Institutionen för kemi- och bioteknik, Industriell Bioteknik )
Bioresource Technology (0960-8524). Vol. 172 (2014), p. 303-311.
[Artikel, refereegranskad vetenskaplig]

Fed-batch simultaneous saccharification and fermentation (SSF) is a feasible option for bioethanol production from lignocellulosic raw materials at high substrate concentrations. In this work, a segregated kinetic model was developed for simulation of fed-batch simultaneous saccharification and co-fermentation (SSCF) of steam-pretreated birch, using substrate, enzymes and cell feeds. The model takes into account the dynamics of the cellulase–cellulose system and the cell population during SSCF, and the effects of pre-cultivation of yeast cells on fermentation performance. The model was cross-validated against experiments using different feed schemes. It could predict fermentation performance and explain observed differences between measured total yeast cells and dividing cells very well. The reproducibility of the experiments and the cell viability were significantly better in fed-batch than in batch SSCF at 15% and 20% total WIS contents. The model can be used for simulation of fed-batch SSCF and optimization of feed profiles.

Nyckelord: 2nd generation biofuel, Fed-batch saccharification and co-fermentation, S. cerevisiae, Enzymatic hydrolysis, Segregated cell model



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Denna post skapades 2014-11-28. Senast ändrad 2015-07-28.
CPL Pubid: 206864

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Industriell Bioteknik (2008-2014)

Ämnesområden

Energi
Livsvetenskaper
Bioenergi

Chalmers infrastruktur