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Protein-tyrosine phosphorylation interaction network in Bacillus subtilis reveals new substrates, kinase activators and kinase cross-talk

Lei Shi (Institutionen för kemi- och bioteknik, Systembiologi) ; N. Pigeonneau ; M. Ventroux ; Abderahmane Derouiche (Institutionen för kemi- och bioteknik, Systembiologi) ; Vladimir Bidnenko (Institutionen för kemi- och bioteknik, Systembiologi) ; I. Mijakovic ; M. F. Noirot-Gros
Frontiers in Microbiology (1664-302X). Vol. 5 (2014), p. Art. no. 538.
[Artikel, refereegranskad vetenskaplig]

Signal transduction in eukaryotes is generally transmitted through phosphorylation cascades that involve a complex interplay of transmembrane receptors, protein kinases, phosphatases and their targets. Our previous work indicated that bacterial protein-tyrosine kinases and phosphatases may exhibit similar properties, since they act on many different substrates. To capture the complexity of this phosphorylation-based network, we performed a comprehensive interactome study focused on the protein-tyrosine kinases and phosphatases in the model bacterium Bacillus subtilis. The resulting network identified many potential new substrates of kinases and phosphatases, some of which were experimentally validated. Our study highlighted the role of tyrosine and serine/threonine kinases and phosphatases in DNA metabolism, transcriptional control and cell division. This interaction network reveals significant crosstalk among different classes of kinases. We found that tyrosine kinases can bind to several modulators, transmembrane or cytosolic, consistent with a branching of signaling pathways. Most particularly, we found that the division site regulator MinD can form a complex with the tyrosine kinase PtkA and modulate its activity in vitro. In vivo, it acts as a scaffold protein which anchors the kinase at the cell pole. This network highlighted a role of tyrosine phosphorylation in the spatial regulation of the Z-ring during cytokinesis.

Nyckelord: protein phosphorylation; protein-protein network; bacterial protein kinase; bacterial cell division; bacterial signaling



Denna post skapades 2014-11-28. Senast ändrad 2014-12-01.
CPL Pubid: 206859

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Systembiologi (2008-2014)

Ämnesområden

Mikrobiologi

Chalmers infrastruktur