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In vitro assessment of platelet concentrates with multiple electrode aggregometry.

Caroline Andersson Shams Hakimi ; Camilla Hesse ; Håkan Wallén ; Fredrik Boulund (Institutionen för matematiska vetenskaper, matematisk statistik) ; Ammi Grahn ; Anders Jeppsson
Platelets (1369-1635). Vol. 26 (2015), 2, p. 132-137.
[Artikel, refereegranskad vetenskaplig]

ABSTRACT Storage impairs platelet function. It was hypothesized that multiple electrode aggregometry in vitro could be used to follow aggregability in platelet concentrates over time and that the results predict the efficacy of platelet transfusion in an ex vivo transfusion model. In vitro platelet aggregability was assessed in apheresis and pooled buffy coat platelet concentrates (BCs) (n = 13 each) using multiple electrode aggregometry with different agonists 1, 3, 5 and 7 days after preparation. In the ex vivo transfusion model, whole blood samples from nine healthy volunteers were collected every second day. The samples were supplemented with stored platelets (+146 × 10(9) × l(-1)) from the same unit 1, 3, 5 and 7 days after preparation. Platelet aggregability was assessed in the concentrate and in the whole blood samples before and after platelet supplementation. There was a continuous reduction in in vitro platelet aggregability over time in both apheresis and pooled BCs. The same pattern was observed after ex vivo addition of apheresis and pooled BCs to whole blood samples. The best correlation between in vitro aggregability and changes in aggregation after addition was achieved with collagen as agonist (r = 0.67, p < 0.001). In conclusion, multiple electrode aggregometry can be used to follow aggregability in platelet concentrates in vitro, and the results predict with moderate accuracy changes in aggregation after addition of platelet concentrate to whole blood samples.

Nyckelord: Aggregometry; apheresis; buffy coat; platelets



Denna post skapades 2014-11-19. Senast ändrad 2015-04-23.
CPL Pubid: 206051

 

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Institutioner (Chalmers)

Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin (GU)
Institutionen för matematiska vetenskaper, matematisk statistik (2005-2016)
Institutionen för medicin, avdelningen för molekylär och klinisk medicin (GU)

Ämnesområden

Klinisk medicin

Chalmers infrastruktur