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Industrial yeasts strains for biorefinery solutions: Constructing and selecting efficient barcoded xylose fermenting strains for ethanol

Elia Tomas-Pejo (Institutionen för kemi- och bioteknik, Industriell Bioteknik ) ; Nicklas Bonander ; Lisbeth Olsson (Institutionen för kemi- och bioteknik, Industriell Bioteknik )
Biofuels, Bioproducts and Biorefining (1932-104X). Vol. 8 (2014), 5, p. 626-634.
[Artikel, refereegranskad vetenskaplig]

There have been intense efforts in the scientific community and industry to engineer S. cerevisiae strains to co-consume xylose and glucose. In this context, this paper describes a process for introducing a barcode into an industrial xylose fermenting yeast strains to ensure quick and reliable identification of patented industrial strains. Furthermore, this work presents a novel procedure for screening and selecting the clones that reflects the characteristics of the industrial process. The key points of efficient selection of high-performing clones are elaborated and discussed. In spite of the unforeseen effects on yeast physiology after the introduction of a new sequence into the genome, we obtained barcoded clones with the same xylose consumption rates and ethanol yields as the cells without the barcode.

Nyckelord: saccharomyces-cerevisiae, lignocellulosic biomass, pluripotential cells



Denna post skapades 2014-11-12. Senast ändrad 2015-03-30.
CPL Pubid: 205673

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Industriell Bioteknik (2008-2014)

Ämnesområden

Bioenergi

Chalmers infrastruktur