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Altered sterol composition renders yeast thermotolerant

Luis Caspeta ; Yun Chen ; Payam Ghiaci (Institutionen för kemi- och bioteknik, Systembiologi) ; Amir Feizi ; S. Buskov ; B. M. Hallstrom ; Dina Petranovic (Institutionen för kemi- och bioteknik, Systembiologi) ; Jens B. Nielsen (Institutionen för kemi- och bioteknik, Systembiologi)
Science (0036-8075). Vol. 346 (2014), 6205, p. 75-78.
[Artikel, refereegranskad vetenskaplig]

Ethanol production for use as a biofuel is mainly achieved through simultaneous saccharification and fermentation by yeast. Operating at >= 40 degrees C would be beneficial in terms of increasing efficiency of the process and reducing costs, but yeast does not grow efficiently at those temperatures. We used adaptive laboratory evolution to select yeast strains with improved growth and ethanol production at >= 40 degrees C. Sequencing of the whole genome, genome-wide gene expression, and metabolic-flux analyses revealed a change in sterol composition, from ergosterol to fecosterol, caused by mutations in the C-5 sterol desaturase gene, and increased expression of genes involved in sterol biosynthesis. Additionally, large chromosome III rearrangements and mutations in genes associated with DNA damage and respiration were found, but contributed less to the thermotolerant phenotype.



Denna post skapades 2014-10-24. Senast ändrad 2015-12-17.
CPL Pubid: 204818

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Systembiologi (2008-2014)

Ämnesområden

Bioenergi

Chalmers infrastruktur

 


Projekt

Denna publikation är ett resultat av följande projekt:


Industrial Systems Biology of Yeast and A. oryzae (INSYSBIO) (EC/FP7/247013)