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Biocompatibility evaluation of densified bacterial nanocellulose hydrogel as an implant material for auricular cartilage regeneration

Héctor Martínez Ávila (Institutionen för kemi- och bioteknik, Polymerteknologi) ; S. Schwarz ; E. M. Feldmann ; Athanasios Mantas (Institutionen för kemi- och bioteknik, Polymerteknologi) ; A. von Bomhard ; Paul Gatenholm (Institutionen för kemi- och bioteknik, Polymerteknologi) ; N. Rotter
Applied Microbiology and Biotechnology (0175-7598). Vol. 98 (2014), 17, p. 7423-7435.
[Artikel, refereegranskad vetenskaplig]

Bacterial nanocellulose (BNC), synthesized by the bacterium Gluconacetobacter xylinus, is composed of highly hydrated fibrils (99 % water) with high mechanical strength. These exceptional material properties make BNC a novel biomaterial for many potential medical and tissue engineering applications. Recently, BNC with cellulose content of 15 % has been proposed as an implant material for auricular cartilage replacement, since it matches the mechanical requirements of human auricular cartilage. This study investigates the biocompatibility of BNC with increased cellulose content (17 %) to evaluate its response in vitro and in vivo. Cylindrical BNC structures (48 Au 20 mm) were produced, purified in a built-in house perfusion system, and compressed to increase the cellulose content in BNC hydrogels. The reduction of endotoxicity of the material was quantified by bacterial endotoxin analysis throughout the purification process. Afterward, the biocompatibility of the purified BNC hydrogels with cellulose content of 17 % was assessed in vitro and in vivo, according to standards set forth in ISO 10993. The endotoxin content in non-purified BNC (2,390 endotoxin units (EU)/ml) was reduced to 0.10 EU/ml after the purification process, level well below the endotoxin threshold set for medical devices. Furthermore, the biocompatibility tests demonstrated that densified BNC hydrogels are non-cytotoxic and cause a minimal foreign body response. In support with our previous findings, this study concludes that BNC with increased cellulose content of 17 % is a promising non-resorbable biomaterial for auricular cartilage tissue engineering, due to its similarity with auricular cartilage in terms of mechanical strength and host tissue response.

Nyckelord: Bacterial cellulose, Depyrogenation, Endotoxin analysis, Biocompatibility, Auricular cartilage tissue, CELLULOSE SCAFFOLDS, ACETOBACTER-XYLINUM, POTENTIAL SCAFFOLD, CHONDROCYTES, INFLAMMATION, RESPONSES, BEHAVIOR, SYSTEMS, PLA

Denna post skapades 2014-10-20. Senast ändrad 2015-03-12.
CPL Pubid: 204570


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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Polymerteknologi (2005-2014)


Medicinska material och protesteknik
Medicinsk bioteknologi

Chalmers infrastruktur

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Biofabrication, Biomechanics and Biocompatibility of Nanocellulose-based Scaffolds for Auricular Cartilage Regeneration