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What do photons do to fluorescently stained DNA in confinement?

J.P. Beech ; Lena Nyberg (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Joachim Fritzsche (Institutionen för teknisk fysik, Kemisk fysik) ; Fredrik Westerlund (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; J. O. Tegenfeldt
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013 Vol. 1 (2014), p. 5-7.
[Konferensbidrag, refereegranskat]

We have studied a selection of factors influencing the damage of DNA in nanochannels during fluorescence imaging. For cutting and nicking of DNA we show that the DNA is shortened during imaging. To avoid photodamage over the course of several hours of a typical experiment, we demonstrate the importance of an oxygen free gas to propel the buffer solution through the device. Finally, by varying the size of the channels, we show indications that higher DNA concentrations lead to higher rates of photodamage necessitating a balance between needs for highly stretched DNA and needs for long measurement times.

Nyckelord: Confinement; DNA; Intercalating dye; Nano-channels; Photobleaching



Denna post skapades 2014-10-08. Senast ändrad 2015-07-28.
CPL Pubid: 203934

 

Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)
Institutionen för teknisk fysik, Kemisk fysik (1900-2015)

Ämnesområden

Fysikalisk kemi
Nanoteknik

Chalmers infrastruktur