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Identifying bacteria using DNA binding maps

Gustav Emilsson (Institutionen för teknisk fysik, Bionanofotonik) ; A. Nilsson ; Lena Nyberg (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; C. Noble ; L. Svensson Stadler ; Edward R.B. Moore ; T. Ambjörnsson ; J.O. Tegenfeldt ; Fredrik Westerlund (Institutionen för kemi- och bioteknik, Fysikalisk kemi)
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013 Vol. 1 (2014), p. 473-475.
[Konferensbidrag, refereegranskat]

We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known sequence. The goal is to create a method for fast identification of bacteria from single DNA molecules without the need for additional cultivation or amplification. We here demonstrate a proof-of-principle experiment on phage DNA and furthermore show that the method can be used to distinguish between two strains of E. coli DNA and to map pieces of DNA onto the full genome.

Nyckelord: Bacterial identification; Nanofluidics; Optical mapping



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Denna post skapades 2014-10-08. Senast ändrad 2016-01-11.
CPL Pubid: 203933

 

Institutioner (Chalmers)

Institutionen för teknisk fysik, Bionanofotonik (2007-2015)
Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)

Ämnesområden

Livsvetenskaper
Nanovetenskap och nanoteknik
Fysikalisk kemi
Nanoteknik

Chalmers infrastruktur