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Photophysics and ex vivo biodistribution of beta-cyclodextrin-meso-tetra(m-hydroxyphenyl)-porphyrin conjugate for biomedical applications

Vladimir Kirejev ; A. R. Goncalves ; C. Aggelidou ; I. Manet ; Jerker Mårtensson (Institutionen för kemi- och bioteknik, Organisk kemi) ; K. Yannakopoulou ; Marica B Ericson
Photochemical & Photobiological Sciences (1474-905X). Vol. 13 (2014), 8, p. 1185-1191.
[Artikel, refereegranskad vetenskaplig]

Low aqueous solubility of porphyrin-based photosensitizers hampers their clinical use in photodynamic therapy because of complex delivery. In this study, we explore meso-tetra(m-hydroxyphenyl)-21,23H-porphyrin (mTHPP), a potent photosensitizer, covalently attached to beta-cyclodextrin (CD-mTHPP) with a focus on topical delivery and cellular uptake. The photophysical properties of CD-mTHPP were examined using steady-state fluorescence and lifetime measurements verifying increased aqueous solubility. Confocal and fluorescence lifetime imaging microscopy on human squamous carcinoma cells (A431) evidenced a cytoplasmic uptake of CD-mTHPP in predominantly monomeric form. CD-mTHPP was also delivered to human skin ex vivo and the skin penetration was assessed using two-photon fluorescence microscopy. The results indicated that CD-mTHPP exhibits improved skin distribution compared to mTHPP alone using aqueous vehicles. Thus the CD-mTHPP conjugate demonstrates improved biodistribution ex vivo compared to mTHPP and is a promising multimodal system for photodynamic therapy.



Denna post skapades 2014-09-30. Senast ändrad 2017-10-03.
CPL Pubid: 203540

 

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Institutioner (Chalmers)

Institutionen för kemi och molekylärbiologi (GU)
Institutionen för kemi- och bioteknik, Organisk kemi (2006-2014)

Ämnesområden

Organisk kemi

Chalmers infrastruktur