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2-Butanol and Butanone Production in Saccharomyces cerevisiae through Combination of a B-12 Dependent Dehydratase and a Secondary Alcohol Dehydrogenase Using a TEV-Based Expression System

Payam Ghiaci (Institutionen för kemi- och bioteknik, Systembiologi) ; Joakim Norbeck (Institutionen för kemi- och bioteknik, Systembiologi) ; Christer Larsson (Institutionen för kemi- och bioteknik, Systembiologi)
Plos One (1932-6203). Vol. 9 (2014), 7,
[Artikel, refereegranskad vetenskaplig]

2-Butanol and its chemical precursor butanone (methyl ethyl ketone -MEK) are chemicals with potential uses as biofuels and biocommodity chemicals. In order to produce 2-butanol, we have demonstrated the utility of using a TEV-protease based expression system to achieve equimolar expression of the individual subunits of the two protein complexes involved in the B-12-dependent dehydratase step (from the pdu-operon of Lactobacillus reuterii), which catalyze the conversion of meso-2,3-butanediol to butanone. We have furthermore identified a NADH dependent secondary alcohol dehydrogenase (Sadh from Gordonia sp.) able to catalyze the subsequent conversion of butanone to 2-butanol. A final concentration of 4 +/- 0.2 mg/L 2-butanol and 2 +/- 0.1 mg/L of butanone was found. A key factor for the production of 2-butanol was the availability of NADH, which was achieved by growing cells lacking the GPD1 and GPD2 isogenes under anaerobic conditions.


Article Number: e102774



Denna post skapades 2014-08-29. Senast ändrad 2014-09-04.
CPL Pubid: 202101

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Systembiologi (2008-2014)

Ämnesområden

Biologisk systematik

Chalmers infrastruktur