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Construction and Modeling of Concatemeric DNA Multilayers on a Planar Surface as Monitored by QCM-D and SPR

Lu Sun (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Sofia Svedhem (Institutionen för teknisk fysik, Biologisk fysik) ; Björn Åkerman (Institutionen för kemi- och bioteknik, Fysikalisk kemi)
Langmuir (0743-7463). Vol. 30 (2014), 28, p. 8432-8441.
[Artikel, refereegranskad vetenskaplig]

The sequential hybridization of a 534 base pair DNA concatemer layer was monitored by QCM-D and SPR, and the QCM-D data were analyzed by Voigt viscoelastic models. The results show that Voigt-based modeling gives a good description of the experimental data but only if shear viscosity and elasticity are allowed to depend on the shear frequency. The derived layer thickness, shear viscosity and elasticity of the growing film give a representation of the DNA film in agreement with known bulk properties of DNA, and reveal a maximum in film viscosity when the molecules in the layer contain 75 base pairs. The experimental data during construction of a 3084 bp DNA concatemer layer were compared to predictions of the QCM-D response of a 1 mu m thick film of rod-like polymers. A predicted nonmonotonous variation of dissipation with frequency (added mass) is in qualitative agreement with the experiments, but with a quantitative disagreement which likely reflects that the flexibility of such long DNA molecules is not included in the model.

Nyckelord: QUARTZ-CRYSTAL MICROBALANCE, PLASMON RESONANCE BIOSENSOR, SUBSEQUENT, HYBRIDIZATION, VISCOELASTIC PROPERTIES, GOLD SURFACES, MOLECULE, IMMOBILIZATION, FILMS, CONFORMATION, SPECTROSCOPY



Denna post skapades 2014-08-28. Senast ändrad 2016-06-30.
CPL Pubid: 202089

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)
Institutionen för teknisk fysik, Biologisk fysik (2007-2015)

Ämnesområden

Biologisk fysik

Chalmers infrastruktur

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