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Identification and qualitative characterization of high and low lignin lines from an oat TILLING population

Vivekanand Vivekanand ; Aakash Chawade ; Mikael Larsson (Institutionen för kemi- och bioteknik, Farmaceutisk teknologi ; SuMo Biomaterials) ; Anette Larsson (Institutionen för kemi- och bioteknik, Farmaceutisk teknologi ; SuMo Biomaterials) ; Olof Olsson
Industrial crops and products (0926-6690). Vol. 59 (2014), p. 1-8.
[Artikel, refereegranskad vetenskaplig]

To identify differences in seed lignin content, 520 randomly chosen independent lines were screened in mutagenized oat population and lines with the seed lignin levels ranging from 20 to 63 gkg(-1) were identified. In the commercial variety Belinda, from which the mutated population was developed, seed lignin level was determined to be 41 g kg(-1). In Assiniboia, a Canadian low lignin variety, it was found to be 21 g kg(-1). To investigate if extracted lignin from the mutated lines were also qualitatively different from Belinda, two lines with the lowest and highest lignin levels were selected for structural analyses using XRD, UV and FT-IR spectroscopy. Results showed that there were significant qualitative differences in seed lignin in the mutated lines and in Belinda, and based on this, we predict that lignin from the mutated seeds will be more digestible in ruminant animals than Belinda seeds. This prediction was confirmed in preliminary in vitro digestion experiments.

Nyckelord: Oat, Lignin, Belinda, Assiniboia, Dioxane, UV, FT-IR spectroscopy



Denna post skapades 2014-08-26. Senast ändrad 2016-09-05.
CPL Pubid: 201958

 

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Institutioner (Chalmers)

Institutionen för biologi och miljövetenskap (GU)
Institutionen för kemi- och bioteknik, Farmaceutisk teknologi (2005-2014)
SuMo Biomaterials

Ämnesområden

Biokemi

Chalmers infrastruktur