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A new approach to measuring vitamin D in human adipose tissue using time-of-flight secondary ion mass spectrometry: A pilot study

Per Malmberg (Institutionen för kemi- och bioteknik, Analytisk kemi) ; Therese Karlsson ; Henrik Svensson ; Malin Lönn ; Nils-Gunnar Carlsson (Institutionen för kemi- och bioteknik, Livsmedelsvetenskap) ; Ann-Sofie Sandberg (Institutionen för kemi- och bioteknik, Livsmedelsvetenskap) ; Eva Jennische ; Amra Osmancevic ; Agneta Holmäng
Journal of Photochemistry and Photobiology. B: Biology (1011-1344). Vol. 138 (2014), p. 295-301.
[Artikel, refereegranskad vetenskaplig]

Circulating concentrations of vitamin D, 25(OH)D, and 1,25(OH)2D are lower in obese than lean individuals, but little is known about the adipose tissue content of these molecules. The aim of this study was to explore the possibility to use time-of-flight secondary ion mass spectrometry (TOF-SIMS) to measure vitamin D and its metabolites in fat tissue in obese and lean subjects. Abdominal subcutaneous adipose tissue (SAT) biopsies were obtained from three lean and three obese women, and paired biopsies SAT and visceral adipose tissue (VAT) were obtained from three obese subjects during gastric bypass surgery. TOF-SIMS was used to measure vitamin D3, 25(OH)D3, and 1,25(OH)2D3 in adipose tissue. We found that vitamin D3, 25(OH)D3, and 1,25(OH)2D3 in adipose tissue can be measured with TOF-SIMS. In adipose tissue, vitamin D3 and its metabolites were located in adipocyte lipid droplets. The content of vitamin D3 (P = 0.006) and 25(OH)D3 (P = 0.018) were lower in SAT in obese compared with lean women. TOF-SIMS has the potential to semi-quantitatively measure vitamin D metabolites in adipose tissue, and offers a possibility to compare vitamin D levels in different depots and groups of individuals. It also gives the opportunity to explore the localization of vitamin D metabolites at a cellular level.

Nyckelord: Adipose tissue, Vitamin D, TOF-SIMS



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Denna post skapades 2014-08-14. Senast ändrad 2015-10-13.
CPL Pubid: 201446

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Analytisk kemi (2006-2014)
Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi (GU)
Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin (GU)
Institutionen för kemi- och bioteknik, Livsmedelsvetenskap (2005-2014)
Institutionen för neurovetenskap och fysiologi (GU)

Ämnesområden

Livsvetenskaper
Dermatologi och venereologi

Chalmers infrastruktur