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Genome-scale analysis of the high-efficient protein secretion system of Aspergillus oryzae

Lifang Liu (Institutionen för kemi- och bioteknik, Systembiologi) ; Amir Feizi (Institutionen för kemi- och bioteknik, Systembiologi) ; Tobias Österlund (Institutionen för kemi- och bioteknik, Systembiologi) ; C. Hjort ; Jens B. Nielsen (Institutionen för kemi- och bioteknik, Systembiologi)
Bmc Systems Biology (1752-0509). Vol. 8 (2014), 73,
[Artikel, refereegranskad vetenskaplig]

Background: The koji mold, Aspergillus oryzae is widely used for the production of industrial enzymes due to its particularly high protein secretion capacity and ability to perform post-translational modifications. However, systemic analysis of its secretion system is lacking, generally due to the poorly annotated proteome. Results: Here we defined a functional protein secretory component list of A. oryzae using a previously reported secretory model of S. cerevisiae as scaffold. Additional secretory components were obtained by blast search with the functional components reported in other closely related fungal species such as Aspergillus nidulans and Aspergillus niger. To evaluate the defined component list, we performed transcriptome analysis on three a-amylase over-producing strains with varying levels of secretion capacities. Specifically, secretory components involved in the ER-associated processes (including components involved in the regulation of transport between ER and Golgi) were significantly up-regulated, with many of them never been identified for A. oryzae before. Furthermore, we defined a complete list of the putative A. oryzae secretome and monitored how it was affected by overproducing amylase. Conclusion: In combination with the transcriptome data, the most complete secretory component list and the putative secretome, we improved the systemic understanding of the secretory machinery of A. oryzae in response to high levels of protein secretion. The roles of many newly predicted secretory components were experimentally validated and the enriched component list provides a better platform for driving more mechanistic studies of the protein secretory pathway in this industrially important fungus.

Nyckelord: Aspergillus oryzae, alpha-amylase, Comparative genomics, Secretory pathway, Transcriptome, ENDOPLASMIC-RETICULUM, TRANSCRIPTIONAL ACTIVATOR, SACCHAROMYCES-CEREVISIAE, FILAMENTOUS FUNGI, GENE-EXPRESSION, STRESS-RESPONSE, AMYLASE GENES, CELL-WALLS, NIGER, INDUCTION, Mathematical & Computational Biology, RISTENSEN T, 1988, BIO-TECHNOLOGY, V6, P1419

Denna post skapades 2014-07-31. Senast ändrad 2015-11-26.
CPL Pubid: 200793


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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Systembiologi (2008-2014)


Bioinformatik och systembiologi

Chalmers infrastruktur

C3SE/SNIC (Chalmers Centre for Computational Science and Engineering)

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Denna publikation ingår i:

Systems Analysis of the Protein Secretory Pathway in Yeast and Human Cells



Denna publikation är ett resultat av följande projekt:

Industrial Systems Biology of Yeast and A. oryzae (INSYSBIO) (EC/FP7/247013)