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Nanosized fibers' effect on adult human articular chondrocytes behavior.

Hanna Stenhamre (Institutionen för kemi- och bioteknik, Polymerteknologi) ; Anna Thorvaldsson (Institutionen för kemi- och bioteknik, Polymerteknologi) ; Lars Enochson ; Pernilla Walkenström ; Anders Lindahl ; Mats Brittberg ; Paul Gatenholm (Institutionen för kemi- och bioteknik, Polymerteknologi)
Materials science & engineering. C, Materials for biological applications (1873-0191). Vol. 33 (2013), 3, p. 1539-1545.
[Artikel, refereegranskad vetenskaplig]

Tissue engineering with chondrogenic cell based therapies is an expanding field with the intention of treating cartilage defects. It has been suggested that scaffolds used in cartilage tissue engineering influence cellular behavior and thus the long-term clinical outcome. The objective of this study was to assess whether chondrocyte attachment, proliferation and post-expansion re-differentiation could be influenced by the size of the fibers presented to the cells in a scaffold. Polylactic acid (PLA) scaffolds with different fiber morphologies were produced, i.e. microfiber (MS) scaffolds as well as nanofiber-coated microfiber scaffold (NMS). Adult human articular chondrocytes were cultured in the scaffolds in vitro up to 28 days, and the resulting constructs were assessed histologically, immunohistochemically, and biochemically. Attachment of cells and serum proteins to the scaffolds was affected by the architecture. The results point toward nano-patterning onto the microfibers influencing proliferation of the chondrocytes, and the overall 3D environment having a greater influence on the re-differentiation. In the efforts of finding the optimal scaffold for cartilage tissue engineering, studies as the current contribute to the knowledge of how to affect and control chondrocytes behavior.

Nyckelord: Adult, Cartilage, Articular, cytology, Cell Adhesion, Cell Count, Cell Differentiation, Cell Proliferation, Cell Shape, Chondrocytes, cytology, ultrastructure, Chondrogenesis, Humans, Immunohistochemistry, Nanofibers, chemistry, ultrastructure, Particle Size, Tissue Scaffolds

Denna post skapades 2014-07-25. Senast ändrad 2016-07-27.
CPL Pubid: 200720


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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Polymerteknologi (2005-2014)
Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin (GU)
Institutionen för kliniska vetenskaper, sektionen för anestesi, biomaterial och ortopedi, Avdelningen för ortopedi (GU)



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