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Phosphorylation of Bacillus subtilis gene regulator AbrB modulates its DNA-binding properties

A. Kobir ; S. Poncet ; V. Bidnenko ; O. Delumeau ; C. Jers ; S. Zouhir ; R. Grenha ; S. Nessler ; P. Noirot ; Ivan Mijakovic (Institutionen för kemi- och bioteknik, Systembiologi)
Molecular Microbiology (0950-382X). Vol. 92 (2014), 5, p. 1129-1141.
[Artikel, refereegranskad vetenskaplig]

AbrB is a global gene regulator involved in transition phase phenomena in Bacillus subtilis. It participates in a complex regulatory network governing the expression of stationary-phase functions. AbrB was previously found to be phosphorylated on serine 86 located close to its C-terminal oligomerization domain. Here we report that AbrB can be phosphorylated by three B. subtilis serine/threonine kinases expressed during the transition and stationary phase: PrkC, PrkD and YabT. Our in vitro findings suggest that AbrB phosphorylation impedes its DNA binding and abolishes binding cooperativity. In vivo we established that a phospho-mimetic mutation abrB S86D leads to a significant loss of AbrB control over several key target functions: exoprotease production, competence development and sporulation. A wider transcriptome analysis of abrBS86D and S86A mutant strains revealed deregulation of a large number of target genes. We therefore propose that AbrB phosphorylation serves as an additional input for fine-tuning the activity of this ambiactive gene regulator.

Nyckelord: TRANSITION-STATE REGULATOR, MYCOBACTERIUM-TUBERCULOSIS, APRE EXPRESSION, PROTEIN-KINASES, TRANSCRIPTION, SPORULATION, PROMOTER, TYROSINE, DOMAIN, SPECIFICITY, eilly M, 1997, JOURNAL OF BACTERIOLOGY, V179, P522, RAUCH MA, 1995, JOURNAL OF BACTERIOLOGY, V177, P6999, RAUCH MA, 1995, MOLECULAR & GENERAL GENETICS, V246, P756, RAUCH MA, 1989, MOLECULAR MICROBIOLOGY, V3, P1203, RAUCH MA, 1989, EMBO JOURNAL, V8, P1615



Denna post skapades 2014-07-22. Senast ändrad 2014-07-22.
CPL Pubid: 200628

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Systembiologi (2008-2014)

Ämnesområden

Biologisk systematik

Chalmers infrastruktur