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Gcn4p and the Crabtree effect of yeast: drawing the causal model of the Crabtree effect in Saccharomyces cerevisiae and explaining evolutionary trade-offs of adaptation to galactose through systems biology

José Luis Martinez Ruiz (Institutionen för kemi- och bioteknik, Systembiologi) ; Sergio Bordel Velasco (Institutionen för kemi- och bioteknik, Systembiologi) ; Kuk-Ki Hong (Institutionen för kemi- och bioteknik, Systembiologi) ; Jens B. Nielsen (Institutionen för kemi- och bioteknik, Systembiologi)
Fems Yeast Research (1567-1356). Vol. 14 (2014), 4, p. 654-662.
[Artikel, refereegranskad vetenskaplig]

By performing an integrated comparative analysis on the physiology and transcriptome of four different S.cerevisiae strains growing on galactose and glucose, it was inferred that the transcription factors Bas1p, Pho2p, and Gcn4p play a central role in the regulatory events causing the Crabtree effect in S.cerevisiae. The analysis also revealed that a point mutation in the RAS2 observed in a galactose-adapted strain causes a lower Crabtree effect and growth rate on glucose by decreasing the activity of Gcn4p while at the same time is at the origin of higher growth rate on galactose due to a lower activity of the transcriptional repressor Sok2p. The role of Gcn4p on the trade-off effect observed on glucose was confirmed experimentally. This was done by showing that the point mutation in RAS2 does not result in a lower growth rate on glucose if it is introduced in a GCN4-negative background.

Nyckelord: Crabtree effect, Gcn4, Bas1, random sampling, flux analysis, TRANSCRIPTION FACTORS, GLUCOSE REPRESSION, PATHWAY, METABOLISM, REGULATOR, NETWORKS, STRAINS

Denna post skapades 2014-07-22. Senast ändrad 2014-10-27.
CPL Pubid: 200627


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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Systembiologi (2008-2014)


Biologisk systematik

Chalmers infrastruktur