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Parvovirus B19 VLP recognizes globoside in supported lipid bilayers

Waqas Nasir ; Jonas Nilsson ; Sigvard Olofsson ; Marta Bally (Institutionen för teknisk fysik, Biologisk fysik) ; Gustaf E Rydell
Virology (0042-6822). Vol. 456 (2014), p. 364-369.
[Artikel, refereegranskad vetenskaplig]

Studies have suggested that the glycosphingolipid globoside (Gb4Cer) is a receptor for human parvovirus B19. Virus-like particles bind to Gb4Cer on thin-layer chromatograms, but a direct interaction between the virus and lipid membrane-associated Gb4Cer has been debated. Here, we characterized the binding of parvovirus B19 VP1/VP2 virus-like particles to glycosphingolipids (i) on thin-layer chromatograms (TLCs) and (ii) incorporated into supported lipid bilayers (SLBs) acting as cell-membrane mimics. The binding specificities of parvovirus B19 determined in the two systems were in good agreement; the VLP recognized both Gb4Cer and the Forssman glycosphingolipid on TLCs and in SLBs compatible with the role of Gb4Cer as a receptor for this virus.

Nyckelord: Parvovirus B19 virus-like particles, Carbohydrate, Glycan, Glycosphingolipids, Glycobiology



Denna post skapades 2014-07-10. Senast ändrad 2014-07-15.
CPL Pubid: 200394

 

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Institutioner (Chalmers)

Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin (GU)
Institutionen för biomedicin, avdelningen för infektionssjukdomar (GU)
Institutionen för teknisk fysik, Biologisk fysik (2007-2015)

Ämnesområden

Biofysik

Chalmers infrastruktur